Only the Z, ZG, ZGN, and ZGC treatments were used in the out-door cultivation. During the out-door experiments, the night-day temperature ranged from −6.6 to 17.5☌ (daily average 3.1 ± 5.3☌) and irradiance ranged from 0 to 2,300 μmol m −2 s −1 (daily average 1,500 ± 1,090 μmol m −2 s −1). In the in-door cultivation, the treatments for nitrogen and carbon sources determination included pure Z medium (control, Z), Z medium + 5% glycerol (ZG), Z medium + 5% glycerol + 50 μM KNO 3 (ZGN), Z medium + 5% glycerol + 200 μM NH 4Cl (ZGA), Z medium + 5% glycerol + 1 mM Na 2CO 3 (ZNC), Z medium + 5% glycerol + 1 mM Na 2CO 3 + 200 μM NH 4Cl (ZGCA) and Z medium + 5% glycerol + 1 mM Na 2CO 3 + 50 μM KNO 3 (ZGCN) and were performed at 15☌ with an irradiance of 75 μmol m −2 s −1. The micro-scale mass cultivation was performed in stable (in-door) and variable (out-door) conditions during winter and/or early spring in the Czech Republic. In this study, we evaluated the growth characteristics in a micro-scale mass cultivation of the Antarctic soil green alga Chlorella mirabilis under different nitrogen and carbon sources followed by analyses of fatty acid contents. Successful adaptation/acclimatization to low temperatures in micro-algae is usually connected with production of specific biotechnologically important compounds. 4Laboratory of Metabolomics and Isotopic Analyses, Global Change Research Centre AS CR, České Budějovice, Czech Republic. 3Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic.2Centre for Phycology, Institute of Botany AS CR, Třeboň, Czech Republic.1Aquatic Environment Management Division, Central Institute of Fisheries Education, Mumbai, India.
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